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Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

Authors
Lien, Truong Thi NgocLam, Tran DaiAn, Vu Thi HongHoang, Tran VinhQuang, Duong TuanKhieu, Dinh QuangTsukahara, ToshifumiLee, Young HoonKim, Jong Seung
Issue Date
15-1월-2010
Publisher
ELSEVIER
Keywords
MWCNTs-doped polypyrrole; Electrochemical DNA biosensor; Quartz crystal microbalance; Electrochemical impedance spectroscopy
Citation
TALANTA, v.80, no.3, pp.1164 - 1169
Indexed
SCIE
SCOPUS
Journal Title
TALANTA
Volume
80
Number
3
Start Page
1164
End Page
1169
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/117161
DOI
10.1016/j.talanta.2009.09.002
ISSN
0039-9140
Abstract
In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (R-ct) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction. (C) 2009 Elsevier B.V. All rights reserved.
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