Cytokine expression in placenta-derived mesenchymal stem cells in patients with pre-eclampsia and normal pregnancies
- Authors
- Hwang, Jong Ha; Lee, Mi Jung; Seok, Oye Sun; Paek, Yu Chin; Cho, Geum Joon; Seol, Hyun Joo; Lee, Jae Kwan; Oh, Min Jeong
- Issue Date
- 1월-2010
- Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Keywords
- Mesenchymal stem cells; Placenta; Amnion; Decidua; Cytokines
- Citation
- CYTOKINE, v.49, no.1, pp.95 - 101
- Indexed
- SCIE
SCOPUS
- Journal Title
- CYTOKINE
- Volume
- 49
- Number
- 1
- Start Page
- 95
- End Page
- 101
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/117213
- DOI
- 10.1016/j.cyto.2009.08.013
- ISSN
- 1043-4666
- Abstract
- Objectives: The aim of this study was to investigate the levels of cytokines in placenta-derived mesenchymal stem cells (MSCs) in normal pregnancies and those with pre-eclampsia. Materials and methods: C5a, CD40 Ligand, G-CSF, GM-CSF, GRO alpha, I-309, sICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1ra, IL-2, IL-4 IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-16, IL-17, IL-17E, IL-23, IL-27, IL-32 alpha, IP-10, I-TAC, MCP-1, MIF, MIP-1 alpha, MIP-1 beta, Serpin E1, RANTES, SDF-1, TNF alpha, and sTREM-1 were measured in mesenchymal stem cells using the human cytokine array panel A. The soluble intracellular adhesion molecule-1 (sICAM-1), stromal-derived factor-1 (SDF-1) and monocyte chemotactic protein-1 (MCP-1) were measured by real-time PCR and confirmed by Western blot analysis. Results: MSCs derived from the deciduas of normal pregnancies had significantly elevated levels of sICAM (p = 0.000) and SDF-1 (p = 0.011), compared to the pregnancies with pre-eclampsia. The level of MCPA in the decidua-derived MSCs was not significantly different. No significant difference was observed between normal and pre-eclamptic pregnancies for the amnion-derived MSCs. Conclusions: The decreased levels of sICAM and SDF-1 found in the decidua-derived MSCs from pre-eclamptic pregnancies might be associated with some of the immunological alterations in pre-eclampsia. (C) 2009 Elsevier Ltd. All rights reserved.
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