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beta-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production
- Authors
- Lee, Sang-Mok; Jin, Li Hua; Kim, Jae Hyun; Han, Sung Ok; Na, Hyon Bin; Hyeon, Taeghwan; Koo, Yoon-Mo; Kim, Jungbae; Lee, Jung-Heon
- Issue Date
- 1월-2010
- Publisher
- SPRINGER
- Keywords
- Cellulosic ethanol; beta-Glucosidase; Enzyme coating; Electrospun nanofibers; Magnetic nanoparticles
- Citation
- BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.33, no.1, pp.141 - 147
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOPROCESS AND BIOSYSTEMS ENGINEERING
- Volume
- 33
- Number
- 1
- Start Page
- 141
- End Page
- 147
- ISSN
- 1615-7591
- Abstract
- beta-Glucosidase (beta G) can relieve the product inhibition of cellobiose in the cellulosic ethanol production by converting cellobiose into glucose. For the potential recycled uses, beta G was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The beta G coating (EC-beta G) was fabricated by crosslinking additional beta G molecules onto covalently attached beta G molecules (CA-beta G) via glutaraldehyde treatment. The initial activity of EC-beta G was 36 times higher than that of CA-beta G. After 20 days of incubation under shaking, CA-beta G and EC-beta G retained 33 and 91% of each initial activity, respectively. Magnetic nanofibers were also used for easy recovery and recycled uses of beta G coating. It is anticipated that the recycled uses of highly active and stable beta G coating can improve the economics of cellulosic ethanol production so long as economical materials are employed as a host of enzyme immobilization.
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