Employment of Hordein Subunit Polymorphisms in Establishing Selection Criteria for High Quality Malting Barley (Hordeum vulgare L.)Employment of Hordein Subunit Polymorphisms in Establishing Selection Criteria for High Quality Malting Barley (Hordeum vulgare L.)
- Other Titles
- Employment of Hordein Subunit Polymorphisms in Establishing Selection Criteria for High Quality Malting Barley (Hordeum vulgare L.)
- Authors
- Yong Jin Lee; Tong Geon Lee; Woong Bae Jeon; 김대연; Min Jeong Hong; 이만보; Jong Nae Hyun; Mi Jung Kim; 서용원; 박철수; 이미자
- Issue Date
- 2010
- Publisher
- 한국작물학회
- Keywords
- malting barley; parameter; protein subunits; quality
- Citation
- Journal of Crop Science and Biotechnology, v.13, no.2, pp.91 - 97
- Indexed
- SCOPUS
KCI
OTHER
- Journal Title
- Journal of Crop Science and Biotechnology
- Volume
- 13
- Number
- 2
- Start Page
- 91
- End Page
- 97
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/117778
- ISSN
- 1975-9479
- Abstract
- Cereal seed storage proteins are encoded by complex multigene families and their subunit profiles are highly related to end-use qualities. Each fraction of albumin and hordein was extracted and its subunit profile was evaluated in related to malt and grain quality parameters. The purpose of this study was to provide selection criteria for high quality malting barley using grain and malt quality parameters and biochemical-genetic information. Grain and malt quality of 13 local adaptability test (LAT) lines were evaluated for malting process. A total of 16 germplasm accessions of high or low seed storage protein content were also evaluated for biochemicalgenetic analysis. The correlation coefficients among quality parameters were analyzed. Several important quality parameters in brewing process showed significant positive or negative correlations. Seed storage protein subunits of albumin and hordein of all tested lines and accessions were evaluated using 12% 1D SDS-PAGE. Scored data of protein subunit's presence or absence was applied to Agglomerative Hierarchical Clustering (AHC) for statistical analysis and showed specific grouping patterns among tested lines.
Clustered lines with subunit information were highly related with agricultural performance and grain and malt qualities. Based on the profiles of seed storage protein subunits, association of hordein subunit of 38, 43, and 65 kDa with high malt scored lines was found.
The obtained results would provide improved selection criteria for high quality malting barley in the malting barley breeding program.
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