Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Edward | - |
dc.contributor.author | Choi, Yoon Young | - |
dc.contributor.author | Jun, Yesl | - |
dc.contributor.author | Chung, Bong Geun | - |
dc.contributor.author | Lee, Sang-Hoon | - |
dc.date.accessioned | 2021-09-08T10:02:14Z | - |
dc.date.available | 2021-09-08T10:02:14Z | - |
dc.date.created | 2021-06-11 | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/118562 | - |
dc.description.abstract | We have developed a multi-layer, microfluidic array platform containing concave microwells and flat cell culture chambers to culture embryonic stem (ES) cells and regulate uniform-sized embryoid body (EB) formation. The main advantage of this platform was that EBs cultured within the concave microwells of a bottom layer were automatically replated into flat cell culture chambers of a top layer, following inversion of the multi-layer microfluidic array platform. This allowed EB formation and EB replating to be controlled simultaneously inside a single microfluidic device without pipette-based manual cell retrieval, a drawback of previous EB culture methods. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | MEDIATED CONTROL | - |
dc.subject | STEM-CELLS | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | DOCKING | - |
dc.title | Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hoon | - |
dc.identifier.doi | 10.1039/c0lc00005a | - |
dc.identifier.scopusid | 2-s2.0-77957682629 | - |
dc.identifier.wosid | 000282314200001 | - |
dc.identifier.bibliographicCitation | LAB ON A CHIP, v.10, no.20, pp.2651 - 2654 | - |
dc.relation.isPartOf | LAB ON A CHIP | - |
dc.citation.title | LAB ON A CHIP | - |
dc.citation.volume | 10 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 2651 | - |
dc.citation.endPage | 2654 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | MEDIATED CONTROL | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | DOCKING | - |
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