Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval
- Authors
- Kang, Edward; Choi, Yoon Young; Jun, Yesl; Chung, Bong Geun; Lee, Sang-Hoon
- Issue Date
- 2010
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- LAB ON A CHIP, v.10, no.20, pp.2651 - 2654
- Indexed
- SCIE
SCOPUS
- Journal Title
- LAB ON A CHIP
- Volume
- 10
- Number
- 20
- Start Page
- 2651
- End Page
- 2654
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/118562
- DOI
- 10.1039/c0lc00005a
- ISSN
- 1473-0197
- Abstract
- We have developed a multi-layer, microfluidic array platform containing concave microwells and flat cell culture chambers to culture embryonic stem (ES) cells and regulate uniform-sized embryoid body (EB) formation. The main advantage of this platform was that EBs cultured within the concave microwells of a bottom layer were automatically replated into flat cell culture chambers of a top layer, following inversion of the multi-layer microfluidic array platform. This allowed EB formation and EB replating to be controlled simultaneously inside a single microfluidic device without pipette-based manual cell retrieval, a drawback of previous EB culture methods.
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Collections - College of Health Sciences > School of Biomedical Engineering > 1. Journal Articles
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