The presence of CD8(+) invariant NKT cells in mice
- Authors
- Lee, Hyunji; Hong, Changwan; Shin, Junghoon; Oh, Soohwan; Jung, Sundo; Park, Yoon-Kyung; Hong, Seokmann; Lee, Gap Ryol; Park, Se-Ho
- Issue Date
- 31-12월-2009
- Publisher
- KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
- Keywords
- antigens, CD1d; CD8-positive T-lymphocytes; alpha-galactosylceramide; mice, transgenic; natural killer T-cells
- Citation
- EXPERIMENTAL AND MOLECULAR MEDICINE, v.41, no.12, pp.866 - 872
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- EXPERIMENTAL AND MOLECULAR MEDICINE
- Volume
- 41
- Number
- 12
- Start Page
- 866
- End Page
- 872
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/118723
- DOI
- 10.3858/emm.2009.41.12.092
- ISSN
- 1226-3613
- Abstract
- Invariant natural killer T (iNKT) cells develop in the thymus upon recognition of CD1d expressed on developing thymocytes. Although CD4 and CD8 coreceptors are not directly involved in the interaction between CD1d and the T cell receptors (TCRs) of iNKT cells, a conspicuous lack of CD8(+) iNKT cells in mice raised the question of whether CD8(+) iNKT cells are excluded due to negative selection during their thymic development, or if there is no lineage commitment for the development of murine CD8(+) iNKT cells. To address this question, we analyzed iNKT cell-specific TCR V alpha 14(+) transgenic mice, where the V alpha 14 transgene forces the generation of iNKT cells. This allows detailed study of the iNKT cell repertoire. We were able to identify CD8(+) iNKT cells which respond to the NKT cell-specific glycolipid ligand alpha-galactosylceramide. Unlike conventional iNKT cells, CD8(+) iNKT cells produce predominantly IFN-gamma but not IL-4 upon antigen stimulation. We also confirmed the presence of CD8(+) iNKT cells in wild type mice. Our results suggest that CD8(+) NKT cells do exist in mice, although their population size is quite small. Their Th1-skewed phenotype might explain why the population size of this subtype needs to be controlled tightly.
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