Effect of Peroxisome Proliferator-Activated Receptor Gamma Agonists on Myofibroblast Differentiation and Collagen Production in Nasal Polyp-Derived Fibroblasts

Abstract

Objectives: We investigated the effect of transforming growth factor-beta 1 (TGF-beta 1) on the differentiation of nasal polyp-derived Fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor gamma (PPAR gamma) agonists on the effects of TGF-beta 1. Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-beta 1 and exposed them to the PPAR gamma agonists ciglitazone and 15-deoxy-Delta-prostaglandin J2. The expression of alpha-smooth muscle actin (alpha-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays. Results: The treatment with TGF-beta 1 increased the expression of alpha-SMA and collagen, and expression was markedly attenuated by the PPAR gamma agonists. Conclusions: PPAR gamma agonists inhibit the differentiation of TGF-beta 1-activated NPDFs and their production of collagen.