Fatty acid profiling and proteomic analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide
- Authors
- Kim, Soo Rin; Kim, Hee Taek; Park, Hee Jung; Kim, Sooah; Choi, Hee Jung; Hwang, Geum-Sook; Yi, Jong Hoon; Ryu, Do Hyun; Kim, Kyoung Heon
- Issue Date
- 15-Sep-2009
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Supercritical carbon dioxide; Salmonella enterica serotype Typhimurium; Sterilization; GC-MS; Two-dimensional electrophoresis
- Citation
- INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, v.134, no.3, pp.190 - 195
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
- Volume
- 134
- Number
- 3
- Start Page
- 190
- End Page
- 195
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/119315
- DOI
- 10.1016/j.ijfoodmicro.2009.06.006
- ISSN
- 0168-1605
- Abstract
- Non-thermal sterilization and microbial inactivation processes are currently receiving much attention in food and pharmaceutical industries. In particular, since supercritical carbon dioxide (SC-CO2) treatment, which is conducted at relatively low temperatures, is considered to be a promising alternative method to replace thermal sterilization processes that cannot be safely used in foods and bioactive materials. Although SC-CO2 has been applied to many microorganisms, the inactivation of microbial cells by SC-CO2 has only been evaluated by using a conventional viable cell count such as a plating method, by which it is not possible to systematically elucidate the microbial cell inactivation process. Therefore, in this study the physiological status of SC-CO2 treated Salmonella enterica serotype Typhimurium was analyzed by using GC-MS analysis fatty acids with principal component analysis and two-dimensional electrophoresis for protein profiling. From the results of these systemic analyses, it was revealed that SC-CO2 caused significant alterations to the profiles of fatty acids and proteins of the cells. (C) 2009 Elsevier B.V. All rights reserved.
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