Synergistic Coordination of Polyethylene Glycol with ClpB/DnaKJE Bichaperone for Refolding of Heat-Denatured Malate Dehydrogenase
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rui Nian | - |
dc.contributor.author | Kim, Duck Sang | - |
dc.contributor.author | Tan, Lihan | - |
dc.contributor.author | Kim, Chan-Wha | - |
dc.contributor.author | Choe, Woo-Seok | - |
dc.date.accessioned | 2021-09-08T15:39:11Z | - |
dc.date.available | 2021-09-08T15:39:11Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-07 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/119698 | - |
dc.description.abstract | The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone-mediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the time point of introducing PEG to the refolding cocktail. The refolding efficiency reached approximately 90% only when PEG was added at the beginning of refolding reaction. The synergistic coordination of an inexpensive refolding additive PEG with the ClpB/DnaKJE bichaperone system may provide an economical route to further enhance the efficacy of ClpB/DnaKJE refolding cocktail approach, facilitating its implementation in large-scale refolding processes. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 1078-1085, 2009 | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | AGGREGATED PROTEINS | - |
dc.subject | CHAPERONE SYSTEM | - |
dc.subject | GROEL-GROES | - |
dc.subject | CLPB | - |
dc.subject | DNAK | - |
dc.subject | SOLUBILIZATION | - |
dc.subject | MECHANISM | - |
dc.subject | GRPE | - |
dc.subject | RENATURATION | - |
dc.subject | INTERMEDIATE | - |
dc.title | Synergistic Coordination of Polyethylene Glycol with ClpB/DnaKJE Bichaperone for Refolding of Heat-Denatured Malate Dehydrogenase | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chan-Wha | - |
dc.identifier.doi | 10.1002/btpr.175 | - |
dc.identifier.wosid | 000269403000021 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY PROGRESS, v.25, no.4, pp.1078 - 1085 | - |
dc.relation.isPartOf | BIOTECHNOLOGY PROGRESS | - |
dc.citation.title | BIOTECHNOLOGY PROGRESS | - |
dc.citation.volume | 25 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1078 | - |
dc.citation.endPage | 1085 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | AGGREGATED PROTEINS | - |
dc.subject.keywordPlus | CHAPERONE SYSTEM | - |
dc.subject.keywordPlus | GROEL-GROES | - |
dc.subject.keywordPlus | CLPB | - |
dc.subject.keywordPlus | DNAK | - |
dc.subject.keywordPlus | SOLUBILIZATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | GRPE | - |
dc.subject.keywordPlus | RENATURATION | - |
dc.subject.keywordPlus | INTERMEDIATE | - |
dc.subject.keywordAuthor | Malate dehydrogenase (MDH) | - |
dc.subject.keywordAuthor | polyethylene glycol (PEG) | - |
dc.subject.keywordAuthor | molecular bichaperone | - |
dc.subject.keywordAuthor | protein refolding | - |
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