Synergistic Coordination of Polyethylene Glycol with ClpB/DnaKJE Bichaperone for Refolding of Heat-Denatured Malate Dehydrogenase
- Authors
- Rui Nian; Kim, Duck Sang; Tan, Lihan; Kim, Chan-Wha; Choe, Woo-Seok
- Issue Date
- 7월-2009
- Publisher
- WILEY
- Keywords
- Malate dehydrogenase (MDH); polyethylene glycol (PEG); molecular bichaperone; protein refolding
- Citation
- BIOTECHNOLOGY PROGRESS, v.25, no.4, pp.1078 - 1085
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY PROGRESS
- Volume
- 25
- Number
- 4
- Start Page
- 1078
- End Page
- 1085
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/119698
- DOI
- 10.1002/btpr.175
- ISSN
- 8756-7938
- Abstract
- The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone-mediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the time point of introducing PEG to the refolding cocktail. The refolding efficiency reached approximately 90% only when PEG was added at the beginning of refolding reaction. The synergistic coordination of an inexpensive refolding additive PEG with the ClpB/DnaKJE bichaperone system may provide an economical route to further enhance the efficacy of ClpB/DnaKJE refolding cocktail approach, facilitating its implementation in large-scale refolding processes. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 1078-1085, 2009
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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