Microbial assimilation of new photosynthate is altered by plant species richness and nitrogen deposition

Abstract

To determine how plant species richness impacts microbial assimilation of new photosynthate, and how this may be modified by atmospheric N deposition, we analyzed the microbial assimilation of recent photosynthate in a 6-year-long field experiment in which plant species richness, atmospheric N deposition, and atmospheric CO(2) concentration were manipulated in concert. The depleted delta(13)C of fumigation CO(2) enabled us to investigate the effect of plant species richness and atmospheric N deposition on the metabolism of soil microbial communities in the elevated CO(2) treatment. To accomplish this, we determined the delta(13)C of bacterial, actinobacterial, and fungal phospholipid fatty acids (PLFAs). In the elevated CO(2) conditions of this study, the delta(13)C of bacterial PLFAs (i15:0, i16:0, 16:1 omega 7c, 16:1 omega 9c, 10Me16:0, and 10Me18:0) and the fungal PLFA 18:1 omega 9c was significantly lower in species-rich plant communities than in species-poor plant communities, indicating that microbial incorporation of new C increased with plant species richness. Despite an increase in plant production, total PLFA decreased under N deposition. Moreover, N deposition also decreased fungal relative abundance in species-rich plant communities. In our study, plant species richness directly increased microbial incorporation of new photosynthate, providing a mechanistic link between greater plant detritus production in species-rich plant communities and larger and more active soil microbial community.