Arsenate tolerance mechanism of Oenothera odorata from a mine population involves the induction of phytochelatins in roots

Abstract

We investigated the arsenate tolerance mechanisms of Oenothera odorata by comparing two populations [i.e., one population from the mine site (MP) and the other population from an uncontaminated site (UP)] via the exposure of hydroponic solution containing arsenate (i.e., 0-50 mu M). The MP plants were significantly more tolerant to arsenate than UP plants. The UP plants accumulated more As in their shoots and roots than did the MP plants. The UP plants translocated up to 21 mu g g(-1) of As into shoots, whereas MP plants translocated less As (up to 4.5 mu g g(-1)) to shoots over all treatments. The results of lipid peroxidation indicated that MP plants were less damaged by oxidative stress than were UP plants. Phytochelatin (PC) content correlated linearly with root As concentration in the MP (i.e., [PCs](root) = 1.69 x [As](root), r(2) = 0.945) and UP (i.e., [PCS](root) = 0.89 x [As](root), r(2) = 0.979) plants. This relationship means that increased PC to As ratio may be associated with increased tolerance. Our results suggest that PC induction in roots plays a critical role in As tolerance of O. odorata. (C) 2008 Elsevier Ltd. All rights reserved.