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Highly Fluorescing Solid DNA-Cationic Polyelectrolyte Complexes Prepared from a Natural DNA and a Poly(fluorenevinylene-alt-phenylene) Bearing Quaternary Ammonium Pendants
- Authors
- Yu, Young-Jun; Kwon, Young-Wan; Kim, Kyu-Nam; Do, Eui-Doo; Choi, Dong-Hoon; Jin, Jung-Il; Shin, Hee-Won; Kim, Yong-Rok; Kang, Ik Joong; Mikroyannidis, John A.
- Issue Date
- Apr-2009
- Publisher
- SPRINGER
- Keywords
- DNA; fluorescence; complexation; polyelectrolyte; conjugated polymer
- Citation
- MACROMOLECULAR RESEARCH, v.17, no.4, pp.245 - 249
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- MACROMOLECULAR RESEARCH
- Volume
- 17
- Number
- 4
- Start Page
- 245
- End Page
- 249
- ISSN
- 1598-5032
- Abstract
- A fluorescing, copolymer (Q)-bearing, quaternary ammonium pendant was mixed with excess natural salmon sperm DNA with a molecular weight of 1.3x10(6)(2,000 base pairs) to afford highly fluorescing, complex mixtures. The fluorescence life-time of the polymer Q was greatly increased when mixed with DNA: for the mixture of Q:DNA=1:750 the fast and slow decay lifetimes increased from ca. 10 to 100 ps and from 20 ps to ca. 1 ns, respectively. The enhanced fluorescence of the mixtures was ascribed to efficient compartmentalization and reduced conformational relaxation of the polymer (c) by complexation with excess DNA.
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