Expression and function of semaphorin 3A and its receptors in human monocyte-derived macrophages
- Authors
- Ji, Jong-Dae; Park-Min, Kyung-Hyun; Ivashkiv, Lionel B.
- Issue Date
- 4월-2009
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Semaphorin; Neuropilin; Plexin; Macrophages
- Citation
- HUMAN IMMUNOLOGY, v.70, no.4, pp.211 - 217
- Indexed
- SCIE
SCOPUS
- Journal Title
- HUMAN IMMUNOLOGY
- Volume
- 70
- Number
- 4
- Start Page
- 211
- End Page
- 217
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120348
- DOI
- 10.1016/j.humimm.2009.01.026
- ISSN
- 0198-8859
- Abstract
- Semaphorins are a large family of secreted and membrane-bound proteins. Recently, several roles of semaphorins in the immune system have emerged. Several semaphorins and their receptors are expressed in a variety of lymphoid and myeloid cells and affect immune cell functions, including cell proliferation, differentiation, chemotaxis, and cytokine production. However, the roles of class 3 semaphorins in human myeloid cells are not well known. Here we examined the regulation of expression of class 3 semaphorins and their receptors by inflammatory stimuli and their function in human macrophages. We show that the expression of Sema3A receptors (neuropilin-1 (NRP-1), NRP-2, plexin A1, plexin A2, and plexin A3) significantly increased during M-CSF-mediated differentiation of monocytes into macrophages under conditions that promote an M2 alternatively activated macrophage phenotype. Consistent with increased NRP-1 expression, cell surface binding of Senna3A increased during M2 differentiation. Interferon (IFN)-gamma and lipopolysaccharide, which promote classical M1 macrophage activation affected expression of NRP-1, NRP-2 and plexin A1. IFN-gamma decreased NRP-1 expression and LPS suppressed NRP-2 and plexin A1 expression. Furthermore we show that Sema3A induced apoptosis in monocyte-derived macrophages and cooperated with anti-Fas CH11 antibody to augment apoptosis. Our results suggest that Sema3A plays a role in induction of apoptosis in monocyte-derived macrophages that are resistant to Fas-induced apoptosis, and that its function can be modulated in inflammatory conditions. (C) 2009 Society. Published by Elsevier Inc. All rights reserved.
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