Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip
- Authors
- Kim, Yeon Seok; Niazi, Javed H.; Gu, Man Bock
- Issue Date
- 23-Feb-2009
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Aptamer; Electrochemical detection; Oxytetracycline; Interdigitated array electrode
- Citation
- ANALYTICA CHIMICA ACTA, v.634, no.2, pp 250 - 254
- Pages
- 5
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICA CHIMICA ACTA
- Volume
- 634
- Number
- 2
- Start Page
- 250
- End Page
- 254
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120565
- DOI
- 10.1016/j.aca.2008.12.025
- ISSN
- 0003-2670
1873-4324
- Abstract
- An electrochemical sensing system for oxytetracycline (OTC) detection was developed using ssDNA aptamer immobilized on gold interdigitated array (IDA) electrode chip. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline JET). The immobilized thiol-modified aptamer on gold electrode chip served as a biorecognition element for the target molecules and the electrochemical signals generated from interactions between the aptamers and the target molecules was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV). The current decrease due to the interference of bound OTC, DOX or TET was analyzed with the electron flow produced by a redox reaction between ferro- and ferricyanide. The specificity of developed EC-biosensor for OTC was highly distinguishable from the structurally similar antibiotics (DOX and TET). The dynamic range was determined to be 1-100 nM of OTC concentration in semi-logarithmic coordinates. (C) 2008 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.