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Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip

Authors
Kim, Yeon SeokNiazi, Javed H.Gu, Man Bock
Issue Date
23-Feb-2009
Publisher
ELSEVIER SCIENCE BV
Keywords
Aptamer; Electrochemical detection; Oxytetracycline; Interdigitated array electrode
Citation
ANALYTICA CHIMICA ACTA, v.634, no.2, pp 250 - 254
Pages
5
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICA CHIMICA ACTA
Volume
634
Number
2
Start Page
250
End Page
254
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/120565
DOI
10.1016/j.aca.2008.12.025
ISSN
0003-2670
1873-4324
Abstract
An electrochemical sensing system for oxytetracycline (OTC) detection was developed using ssDNA aptamer immobilized on gold interdigitated array (IDA) electrode chip. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline JET). The immobilized thiol-modified aptamer on gold electrode chip served as a biorecognition element for the target molecules and the electrochemical signals generated from interactions between the aptamers and the target molecules was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV). The current decrease due to the interference of bound OTC, DOX or TET was analyzed with the electron flow produced by a redox reaction between ferro- and ferricyanide. The specificity of developed EC-biosensor for OTC was highly distinguishable from the structurally similar antibiotics (DOX and TET). The dynamic range was determined to be 1-100 nM of OTC concentration in semi-logarithmic coordinates. (C) 2008 Elsevier B.V. All rights reserved.
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