Released exopolysaccharide (r-EPS) produced from probiotic bacteria reduce biofilm formation of enterohemorrhagic Escherichia coli O157:H7
- Authors
- Kim, Younghoon; Oh, Sejong; Kim, Sae Hun
- Issue Date
- 6-Feb-2009
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- EHEC O157:H7; Lactobacillus acidophilus; Biofilm formation; Exopolysaccharide
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.379, no.2, pp.324 - 329
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 379
- Number
- 2
- Start Page
- 324
- End Page
- 329
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120588
- DOI
- 10.1016/j.bbrc.2008.12.053
- ISSN
- 0006-291X
- Abstract
- Here, we characterized released-exopolysaccharides (r-EPS) from Lactobacillus acidophilus A4 with the goal of identifying natural compounds that represses biofilm formation. In plastic 96-well microplates that contained 1.0 mg/ml of r-EPS, enterohemorrhagic Escherichia coli (EHEC) biofilms were dramatically decreased by 87% and 94% on polystyrene and polyvinyl chloride (PVC) surfaces, respectively. In the presence of r-EPS, neither their growth rate nor their autoinducer-2-like activity was affected on the EHEC O157:H7. Importantly, consistent reduction in biofilm formation was also observed when r-EPS was applied to the continuous-flow chamber models. In addition, we found that adding r-EPS significantly repressed biofilm formation by affecting genes related to curli production (crl, csgA. and csgB) and chemotaxis (cheY) in transcriptome analysis. Furthermore, these r-EPS Could prevent biofilm formation by a wide range of Gram-negative and -positive pathogens. This property may lead to the development of novel food-grade adjuncts for microbial biofilm control. (c) 2008 Elsevier Inc. All rights reserved.
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