An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid in Streptomyces clavuligerus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sae-Jin | - |
dc.contributor.author | Kim, Jin-Oh | - |
dc.contributor.author | Shin, Chang-Hun | - |
dc.contributor.author | Park, Hye Won | - |
dc.contributor.author | Kim, Chan-Wha | - |
dc.date.accessioned | 2021-09-08T21:02:08Z | - |
dc.date.available | 2021-09-08T21:02:08Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2009-01 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120805 | - |
dc.description.abstract | To develop a strategy for improved production of clavulanic acid (CA), we investigated the effect of using oils on cell growth and CA production during the fermentation of Streptomyces clavuligerus NRRL 3585. In this analysis, triolein, whose fatty acid is oleic acid only, was the best oil source for CA production, but free fatty acids generated from the hydrolysis of oils in a culture broth negatively impacted CA production and cell growth. Hence, we screened for mutants that were resistant to high concentrations of oleic acid. From this screen we identified a mutant S. clavuligerus, OLD, that had a minimum inhibitory concentration (MIC) to oleic acid of 2.1 g/l, much higher than that of S. clavuligerus NRRL 3585, at 0.4g/l. Not only was cell growth improved, but maximum CA production, at 1,950 mg/l, was approximately 2.0-fold higher than that of the parent strain. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.subject | SOYBEAN DERIVATIVES | - |
dc.subject | CEPHAMYCIN-C | - |
dc.subject | BETA-LACTAM | - |
dc.subject | FATTY-ACIDS | - |
dc.subject | GENE | - |
dc.subject | OIL | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | OVERPRODUCTION | - |
dc.title | An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid in Streptomyces clavuligerus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chan-Wha | - |
dc.identifier.doi | 10.1271/bbb.80569 | - |
dc.identifier.scopusid | 2-s2.0-58849115023 | - |
dc.identifier.wosid | 000263133400025 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.73, no.1, pp.160 - 164 | - |
dc.relation.isPartOf | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.title | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.volume | 73 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 160 | - |
dc.citation.endPage | 164 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | SOYBEAN DERIVATIVES | - |
dc.subject.keywordPlus | CEPHAMYCIN-C | - |
dc.subject.keywordPlus | BETA-LACTAM | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | OIL | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | OVERPRODUCTION | - |
dc.subject.keywordAuthor | clavulanic acid | - |
dc.subject.keywordAuthor | Streptomyces clavuligerus | - |
dc.subject.keywordAuthor | oleic acid | - |
dc.subject.keywordAuthor | triolein | - |
dc.subject.keywordAuthor | fermentation | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.