An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid in Streptomyces clavuligerus
- Authors
- Kim, Sae-Jin; Kim, Jin-Oh; Shin, Chang-Hun; Park, Hye Won; Kim, Chan-Wha
- Issue Date
- 1월-2009
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- clavulanic acid; Streptomyces clavuligerus; oleic acid; triolein; fermentation
- Citation
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.73, no.1, pp.160 - 164
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Volume
- 73
- Number
- 1
- Start Page
- 160
- End Page
- 164
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120805
- DOI
- 10.1271/bbb.80569
- ISSN
- 0916-8451
- Abstract
- To develop a strategy for improved production of clavulanic acid (CA), we investigated the effect of using oils on cell growth and CA production during the fermentation of Streptomyces clavuligerus NRRL 3585. In this analysis, triolein, whose fatty acid is oleic acid only, was the best oil source for CA production, but free fatty acids generated from the hydrolysis of oils in a culture broth negatively impacted CA production and cell growth. Hence, we screened for mutants that were resistant to high concentrations of oleic acid. From this screen we identified a mutant S. clavuligerus, OLD, that had a minimum inhibitory concentration (MIC) to oleic acid of 2.1 g/l, much higher than that of S. clavuligerus NRRL 3585, at 0.4g/l. Not only was cell growth improved, but maximum CA production, at 1,950 mg/l, was approximately 2.0-fold higher than that of the parent strain.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.