A DNA adjuvant encoding a fusion protein between anti-CD3 single-chain Fv and AIMP1 enhances T helper type 1 cell-mediated immune responses in antigen-sensitized mice
DC Field | Value | Language |
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dc.contributor.author | Lee, Byeong Cheol | - |
dc.contributor.author | O'Sullivan, Insug | - |
dc.contributor.author | Kim, Eugene | - |
dc.contributor.author | Park, Sang Gyu | - |
dc.contributor.author | Hwang, Seung Yong | - |
dc.contributor.author | Cho, Daeho | - |
dc.contributor.author | Kim, Tae Sung | - |
dc.date.accessioned | 2021-09-08T21:15:07Z | - |
dc.date.available | 2021-09-08T21:15:07Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2009-01 | - |
dc.identifier.issn | 0019-2805 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/120873 | - |
dc.description.abstract | T helper type 1 (Th1) cell-mediated immune responses contribute to host defences against intracellular pathogen infections and cancer. Previously, we found that aminoacyl tRNA synthetase-interacting multifunctional protein 1 (AIMP1) activated macrophages and dendritic cells to enhance Th1 responses. Herein, we manipulated this property to improve Th1 immune responses in vivo by constructing a mammalian expression plasmid (pAnti-CD3sFv/AIMP1) encoding AIMP1 fused to the anti-CD3 single-chain Fv (sFv), the smallest unit of the antibody that interacts with the CD3 epsilon region of the T-cell receptor. Intramuscular injection of ovalbumin (OVA)-sensitized BALB/c mice with pAnti-CD3sFv/AIMP1 DNA adjuvant increased the OVA-specific, interferon-gamma production by their CD4(+) T cells and the levels of anti-OVA immunoglobulin G2a (IgG2a) isotype in their sera. Furthermore, the pAnti-CD3sFv/AIMP1 DNA adjuvant decreased interleukin-4 production and anti-OVA IgE levels in the OVA-injected mice. Importantly, the pAnti-CD3sFv/AIMP1 was more efficient than a mixture of pAnti-CD3sFv and pAIMP1 in inducing OVA-specific Th1 immune responses and also in inhibiting OVA-specific Th2 responses during antigen priming. These studies indicated that the pAnti-CD3sFv/AIMP1 fusion DNA adjuvant enhanced Th1 immune responses in antigen-sensitized mice. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | MOLECULAR-MECHANISMS | - |
dc.subject | INTERFERON-GAMMA | - |
dc.subject | VACCINES | - |
dc.subject | IL-12 | - |
dc.subject | INTERLEUKIN-18 | - |
dc.subject | IMMUNOTHERAPY | - |
dc.subject | MACROPHAGES | - |
dc.subject | VACCINATION | - |
dc.subject | ACTIVATION | - |
dc.title | A DNA adjuvant encoding a fusion protein between anti-CD3 single-chain Fv and AIMP1 enhances T helper type 1 cell-mediated immune responses in antigen-sensitized mice | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Sung | - |
dc.identifier.doi | 10.1111/j.1365-2567.2008.02880.x | - |
dc.identifier.scopusid | 2-s2.0-57449111275 | - |
dc.identifier.wosid | 000261528300010 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY, v.126, no.1, pp.84 - 91 | - |
dc.relation.isPartOf | IMMUNOLOGY | - |
dc.citation.title | IMMUNOLOGY | - |
dc.citation.volume | 126 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 84 | - |
dc.citation.endPage | 91 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | MOLECULAR-MECHANISMS | - |
dc.subject.keywordPlus | INTERFERON-GAMMA | - |
dc.subject.keywordPlus | VACCINES | - |
dc.subject.keywordPlus | IL-12 | - |
dc.subject.keywordPlus | INTERLEUKIN-18 | - |
dc.subject.keywordPlus | IMMUNOTHERAPY | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | VACCINATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | aminoacyl t-RNA synthetase-interacting multifunctional protein 1 | - |
dc.subject.keywordAuthor | anti-CD3 | - |
dc.subject.keywordAuthor | DNA adjuvant | - |
dc.subject.keywordAuthor | T cell | - |
dc.subject.keywordAuthor | T helper cell response | - |
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