A DNA adjuvant encoding a fusion protein between anti-CD3 single-chain Fv and AIMP1 enhances T helper type 1 cell-mediated immune responses in antigen-sensitized mice
- Authors
- Lee, Byeong Cheol; O'Sullivan, Insug; Kim, Eugene; Park, Sang Gyu; Hwang, Seung Yong; Cho, Daeho; Kim, Tae Sung
- Issue Date
- 1월-2009
- Publisher
- WILEY
- Keywords
- aminoacyl t-RNA synthetase-interacting multifunctional protein 1; anti-CD3; DNA adjuvant; T cell; T helper cell response
- Citation
- IMMUNOLOGY, v.126, no.1, pp.84 - 91
- Indexed
- SCIE
SCOPUS
- Journal Title
- IMMUNOLOGY
- Volume
- 126
- Number
- 1
- Start Page
- 84
- End Page
- 91
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/120873
- DOI
- 10.1111/j.1365-2567.2008.02880.x
- ISSN
- 0019-2805
- Abstract
- T helper type 1 (Th1) cell-mediated immune responses contribute to host defences against intracellular pathogen infections and cancer. Previously, we found that aminoacyl tRNA synthetase-interacting multifunctional protein 1 (AIMP1) activated macrophages and dendritic cells to enhance Th1 responses. Herein, we manipulated this property to improve Th1 immune responses in vivo by constructing a mammalian expression plasmid (pAnti-CD3sFv/AIMP1) encoding AIMP1 fused to the anti-CD3 single-chain Fv (sFv), the smallest unit of the antibody that interacts with the CD3 epsilon region of the T-cell receptor. Intramuscular injection of ovalbumin (OVA)-sensitized BALB/c mice with pAnti-CD3sFv/AIMP1 DNA adjuvant increased the OVA-specific, interferon-gamma production by their CD4(+) T cells and the levels of anti-OVA immunoglobulin G2a (IgG2a) isotype in their sera. Furthermore, the pAnti-CD3sFv/AIMP1 DNA adjuvant decreased interleukin-4 production and anti-OVA IgE levels in the OVA-injected mice. Importantly, the pAnti-CD3sFv/AIMP1 was more efficient than a mixture of pAnti-CD3sFv and pAIMP1 in inducing OVA-specific Th1 immune responses and also in inhibiting OVA-specific Th2 responses during antigen priming. These studies indicated that the pAnti-CD3sFv/AIMP1 fusion DNA adjuvant enhanced Th1 immune responses in antigen-sensitized mice.
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