Transcriptome analysis of the zebrafish mind bomb mutant

Abstract

Mind bomb (Mib) facilitates Notch signaling pathway by promoting the endocytosis of Notch ligand. The zebrafish mtb(ta52b) mutant has a defect in its ubiquitin ligase activity which is necessary to inhibit the neurogenesis, resulting in a neuronal hyperplasia. Several genes regulated in the mib(ta52b) mutant have been well established, however, there were relatively few reports about the transcriptome profile. To identify the genes differentially expressed in the mib(ta52b) mutant, genome-wide analysis was performed using serial analysis of gene expression. Three hundred and thirty-five transcripts were identified whose expressions were significantly altered in the mib(ta52b) mutant as compared with the wild-type. Interestingly, it was suggested that the mib(ta52b) mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway.