발생 중인 백서 신장에서 Angiotensin Ⅱ 억제가 epithelial to mesenchymal transition에 미치는 효과Effect of angiotensin Ⅱ inhibition on the epithelial to mesenchymal transition in developing rat kidney
- Other Titles
- Effect of angiotensin Ⅱ inhibition on the epithelial to mesenchymal transition in developing rat kidney
- Authors
- 임형은; 배인순; 홍영숙; 이주원; 유기환
- Issue Date
- 2009
- Publisher
- 대한소아청소년과학회
- Keywords
- Angiotensin II; Cell Transdifferentiation; Growth and Development; Kidney Diseases; Angiotensin II; Cell Transdifferentiation; Growth and Development; Kidney Diseases
- Citation
- Clinical and Experimental Pediatrics, v.52, no.8, pp.944 - 952
- Journal Title
- Clinical and Experimental Pediatrics
- Volume
- 52
- Number
- 8
- Start Page
- 944
- End Page
- 952
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/121012
- Abstract
- Purpose : To investigate the effects of angiotensin II inhibition on the epithelial to mesenchymal transition (EMT) in the developing kidney, we tested the expression of EMT markers and nestin in angiotensin converting enzyme (ACE) inhibitor-treated kidneys.
Methods : Newborn rat pups were treated with enalapril (30 mg/kg/d) or a vehicle for 7 days. Immunohistochemistry for the expression of α-smooth muscle actin (SMA), E-cadherin, vimentin, and nestin were performed. The number of positively-stained cells was determined under 100 magnification in 10 random fields.
Results : In the enalapril-treated group, αSMA-positive cells were strongly expressed in the dilated tubular epithelial cells. The number of αSMA-positive cells in the enalapril-treated group increased in both the renal cortex and medulla, compared to the control group (P<0.05). The expression of E-cadherin-positive cells was dramatically reduced in the cortical and medullary tubular epithelial cells in the enalapril-treated group (P<0.05). The number of vimentin- and nestin-positive cells in the cortex was not different in comparisons between the two groups; however, their expression increased in the medullary tubulointerstitial cells in the enalapril-treated group (P<0.05).
Conclusion : Our results show that ACE inhibition in the developing kidney increases the renal EMT by up-regulating αSMA and down-regulating E-cadherin. Enalapril treatment was associated with increased expression of vimentin and nestin in the renal medulla, suggesting that renal medullary changes during the EMT might be more prominent, and ACE inhibition might differentially modulate the expression of EMT markers in the developing rat kidney.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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