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Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method
- Issue Date
- 18-11월-2008
- Publisher
- NATL ACAD SCIENCES
- Keywords
- directed evolution; enzyme design; molecular modeling; redox enzymes; stereoselectivity
- Citation
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.105, no.46, pp.17694 - 17699
- Indexed
- SCIE
SCOPUS
- Volume
- 105
- Number
- 46
- Start Page
- 17694
- End Page
- 17699
- ISSN
- 0027-8424
- Abstract
- A highly efficient selection method for enhanced enzyme enantioselectivity based on yeast surface display and fluorescence-activated cell sorting (FACS) is developed and validated. its application to horseradish peroxidase has resulted in enzyme variants up to 2 orders of magnitude selective toward either substrate enantiomer at will. These marked improvements in enantioselectivity are demonstrated for the surface-bound and soluble enzymes and rationalized by computational docking studies.
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