Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium
- Authors
- Lee, Yong Kwon; Kim, Seung Yeul; Kim, Ki Heon; Chun, Bok-Hwan; Lee, Kweon-Haeng; Oh, Duk Jae; Chung, Namhyun
- Issue Date
- 11월-2008
- Publisher
- SPRINGER
- Keywords
- soybean protein hydrolysates; cell growth; keratinocytes; serum-free medium
- Citation
- BIOTECHNOLOGY LETTERS, v.30, no.11, pp.1931 - 1936
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY LETTERS
- Volume
- 30
- Number
- 11
- Start Page
- 1931
- End Page
- 1936
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122474
- DOI
- 10.1007/s10529-008-9796-0
- ISSN
- 0141-5492
- Abstract
- Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biosystems and Biotechnology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholar.korea.ac.kr/handle/2021.sw.korea/122474)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.