Enhanced Production of 1,2-Propanediol by tpil Deletion in Saccharomyces cerevisiae
- Authors
- Jung, Joon-Young; Choi, Eun-Sil; Oh, Min-Kyu
- Issue Date
- 11월-2008
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Saccharomyces cerevisiae; triosephosphate isomerase; 1,2-propanediol; metabolic engineering
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.11, pp.1797 - 1802
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 18
- Number
- 11
- Start Page
- 1797
- End Page
- 1802
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122512
- DOI
- 10.4014/jmb.0800.010
- ISSN
- 1017-7825
- Abstract
- Saccharomyces cerevisiae was metabolically engineered to improve 1,2-propanediol production. Deletion of the tpil (triosephosphate isomerase) gene in S. cerevisiae increased the carbon flux to DHAP (dihydroxylacetone phosphate) in glycolysis, resulting in increased glycerol production. Then, the mgs and gldA genes, the products of which convert DHAP to 1,2-propanediol, were introduced to the tpil-deficient strain using a multicopy plasmid. As expected, the intracellular level of methylglyoxal was increased by introduction of the mgs gene in S. cerevisiae and that of 1,2-propanediol by introduction of both the mgs and gldA genes. As a result, 1.11 g/l of 1,2-propanediol was achieved in flask culture.
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