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Sample preparation method for plasma membrane proteome analysis

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dc.contributor.authorPark, Seung-Ah-
dc.contributor.authorKim, Mi-Ryung-
dc.contributor.authorKim, Pan-Kyeom-
dc.contributor.authorCho, Hyung-Don-
dc.contributor.authorHan, Gi-Yeon-
dc.contributor.authorKim, Chan-Wha-
dc.date.accessioned2021-09-09T04:30:39Z-
dc.date.available2021-09-09T04:30:39Z-
dc.date.created2021-06-10-
dc.date.issued2008-09-01-
dc.identifier.issn1570-0232-
dc.identifier.urihttps://scholar.korea.ac.kr/handle/2021.sw.korea/122732-
dc.description.abstractThe preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis. (c) 2008 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.language.isoen-
dc.publisherELSEVIER SCIENCE BV-
dc.subjectGEL-ELECTROPHORESIS-
dc.subject2-PHASE PARTITION-
dc.subjectPROTEINS-
dc.subjectEXTRACTION-
dc.subjectSEPARATION-
dc.subjectCELLS-
dc.titleSample preparation method for plasma membrane proteome analysis-
dc.typeArticle-
dc.contributor.affiliatedAuthorKim, Chan-Wha-
dc.identifier.doi10.1016/j.jchromb.2008.07.028-
dc.identifier.scopusid2-s2.0-49849096458-
dc.identifier.wosid000259764800024-
dc.identifier.bibliographicCitationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.872, no.1-2, pp.177 - 180-
dc.relation.isPartOfJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES-
dc.citation.titleJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES-
dc.citation.volume872-
dc.citation.number1-2-
dc.citation.startPage177-
dc.citation.endPage180-
dc.type.rimsART-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalWebOfScienceCategoryBiochemical Research Methods-
dc.relation.journalWebOfScienceCategoryChemistry, Analytical-
dc.subject.keywordPlusGEL-ELECTROPHORESIS-
dc.subject.keywordPlus2-PHASE PARTITION-
dc.subject.keywordPlusPROTEINS-
dc.subject.keywordPlusEXTRACTION-
dc.subject.keywordPlusSEPARATION-
dc.subject.keywordPlusCELLS-
dc.subject.keywordAuthorhMSCs-
dc.subject.keywordAuthorPercoll-
dc.subject.keywordAuthorplasma membrane-
dc.subject.keywordAuthortwo-phase extraction-
dc.subject.keywordAuthortwo-dimensional electrophoresis-
dc.subject.keywordAuthorUltracentrifugation-
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