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Sample preparation method for plasma membrane proteome analysis

Authors
Park, Seung-AhKim, Mi-RyungKim, Pan-KyeomCho, Hyung-DonHan, Gi-YeonKim, Chan-Wha
Issue Date
1-9월-2008
Publisher
ELSEVIER SCIENCE BV
Keywords
hMSCs; Percoll; plasma membrane; two-phase extraction; two-dimensional electrophoresis; Ultracentrifugation
Citation
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.872, no.1-2, pp.177 - 180
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume
872
Number
1-2
Start Page
177
End Page
180
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/122732
DOI
10.1016/j.jchromb.2008.07.028
ISSN
1570-0232
Abstract
The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis. (c) 2008 Elsevier B.V. All rights reserved.
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