Differentiation of endothelial cells derived from mouse embryoid bodies: A possible in vitro vasculogenesis model
DC Field | Value | Language |
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dc.contributor.author | Kim, Gi Dae | - |
dc.contributor.author | Kim, Gi Jin | - |
dc.contributor.author | Seok, Ji Hyun | - |
dc.contributor.author | Chung, Hyung-Min | - |
dc.contributor.author | Chee, Kew-Mahn | - |
dc.contributor.author | Rhee, Gyu-Seek | - |
dc.date.accessioned | 2021-09-09T04:58:15Z | - |
dc.date.available | 2021-09-09T04:58:15Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-08-28 | - |
dc.identifier.issn | 0378-4274 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122844 | - |
dc.description.abstract | Mouse embryonic stern cells (mES cells), which are pluripotent and self-renewal cells, are derived from the inner cell mass of mouse blastocysts. The objective of this study was to construct more efficient mES cell-derived embryoid bodies (EBs) for use as a vasculogenesis model and as an in vitro vascular toxicity testing model. EBs were formed for 3 days using hanging drop cultures and plated on gelatin-coated plates in endothelial growth medium-2 (EGM-2) to promote vascular development. The differentiation of mES cell-derived EBs was confirmed by reverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry, and flow cytometry within 7 days after plating EBs. The mRNA and protein expressions of vascular endothelial growth factor receptors-2 (FLK-1), platelet endothelial cell adhesion molecule (PECAM), and vascular endothelial-cadherin (VE-cadherin) were observed in differentiated mES cells. When placed in matrigel, mES cell-derived endothelial like cells formed networks Similar to vascular structures. mES cells were also exposed to 5-fluorouracil (5-FU). a strong inhibitor of vessel formation, and its cytotoxicity was determined using MTT assays. The inhibitory concentrations (IC50) of 5-FU for mES cells and C166 cells were 0.72 mu M arid 1.04 mu M, respectively. These results demonstrate that mES cells can be used to study vasculogenesis arid for cytotoxicity screening. (C) 2008 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | STEM-CELLS | - |
dc.subject | ADHESION MOLECULE | - |
dc.subject | YOLK-SAC | - |
dc.subject | EXPRESSION | - |
dc.subject | GROWTH | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | BLOOD | - |
dc.subject | ASSAY | - |
dc.subject | PECAM-1/CD31 | - |
dc.subject | BLASTOCYST | - |
dc.title | Differentiation of endothelial cells derived from mouse embryoid bodies: A possible in vitro vasculogenesis model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chee, Kew-Mahn | - |
dc.identifier.doi | 10.1016/j.toxlet.2008.05.023 | - |
dc.identifier.scopusid | 2-s2.0-49549111794 | - |
dc.identifier.wosid | 000259461000002 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY LETTERS, v.180, no.3, pp.166 - 173 | - |
dc.relation.isPartOf | TOXICOLOGY LETTERS | - |
dc.citation.title | TOXICOLOGY LETTERS | - |
dc.citation.volume | 180 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 166 | - |
dc.citation.endPage | 173 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | ADHESION MOLECULE | - |
dc.subject.keywordPlus | YOLK-SAC | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | BLOOD | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | PECAM-1/CD31 | - |
dc.subject.keywordPlus | BLASTOCYST | - |
dc.subject.keywordAuthor | mouse embryonic stern cells | - |
dc.subject.keywordAuthor | vasculogenesis | - |
dc.subject.keywordAuthor | differentiation | - |
dc.subject.keywordAuthor | proliferation | - |
dc.subject.keywordAuthor | cytotoxicity | - |
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