Differentiation of endothelial cells derived from mouse embryoid bodies: A possible in vitro vasculogenesis model
- Authors
- Kim, Gi Dae; Kim, Gi Jin; Seok, Ji Hyun; Chung, Hyung-Min; Chee, Kew-Mahn; Rhee, Gyu-Seek
- Issue Date
- 28-8월-2008
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- mouse embryonic stern cells; vasculogenesis; differentiation; proliferation; cytotoxicity
- Citation
- TOXICOLOGY LETTERS, v.180, no.3, pp.166 - 173
- Indexed
- SCIE
SCOPUS
- Journal Title
- TOXICOLOGY LETTERS
- Volume
- 180
- Number
- 3
- Start Page
- 166
- End Page
- 173
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122844
- DOI
- 10.1016/j.toxlet.2008.05.023
- ISSN
- 0378-4274
- Abstract
- Mouse embryonic stern cells (mES cells), which are pluripotent and self-renewal cells, are derived from the inner cell mass of mouse blastocysts. The objective of this study was to construct more efficient mES cell-derived embryoid bodies (EBs) for use as a vasculogenesis model and as an in vitro vascular toxicity testing model. EBs were formed for 3 days using hanging drop cultures and plated on gelatin-coated plates in endothelial growth medium-2 (EGM-2) to promote vascular development. The differentiation of mES cell-derived EBs was confirmed by reverse transcription-polymerase chain reaction (RT-PCR), immunocytochemistry, and flow cytometry within 7 days after plating EBs. The mRNA and protein expressions of vascular endothelial growth factor receptors-2 (FLK-1), platelet endothelial cell adhesion molecule (PECAM), and vascular endothelial-cadherin (VE-cadherin) were observed in differentiated mES cells. When placed in matrigel, mES cell-derived endothelial like cells formed networks Similar to vascular structures. mES cells were also exposed to 5-fluorouracil (5-FU). a strong inhibitor of vessel formation, and its cytotoxicity was determined using MTT assays. The inhibitory concentrations (IC50) of 5-FU for mES cells and C166 cells were 0.72 mu M arid 1.04 mu M, respectively. These results demonstrate that mES cells can be used to study vasculogenesis arid for cytotoxicity screening. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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