Interactome analysis of yeast glutathione peroxidase 3
- Authors
- Lee, Phil Young; Bae, Kwang-Hee; Kho, Chang Won; Kang, Sunghyun; Lee, Do Hee; Cho, Sayeon; Kang, Seongman; Lee, Sang Chul; Park, Byoung Chul; Park, Sung Goo
- Issue Date
- 8월-2008
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- glutathione peroxidase 3; IP-2DE; oxidative stress; ROS
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.8, pp.1364 - 1367
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 18
- Number
- 8
- Start Page
- 1364
- End Page
- 1367
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122906
- ISSN
- 1017-7825
- Abstract
- Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in V. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.
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Collections - Graduate School > Department of Life Sciences > 1. Journal Articles
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