A comparison of gene expression profiles between primary human AML cells and AML cell line
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jinseok | - |
dc.contributor.author | Hwang, Junmo | - |
dc.contributor.author | Kim, Hyung-Soo | - |
dc.contributor.author | Kim, Seonggon | - |
dc.contributor.author | Kim, Young Hun | - |
dc.contributor.author | Park, So-Young | - |
dc.contributor.author | Kim, Kil Soo | - |
dc.contributor.author | Ryoo, Zae Young | - |
dc.contributor.author | Chang, Kyu-Tae | - |
dc.contributor.author | Lee, Sanggyu | - |
dc.date.accessioned | 2021-09-09T05:38:24Z | - |
dc.date.available | 2021-09-09T05:38:24Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-08 | - |
dc.identifier.issn | 1341-7568 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/122938 | - |
dc.description.abstract | In acute myeloid leukemia (AML), hematologic malignancies are characterized by recurring chromosomal abnormalities. Chromosome translocation t(9;11)(p22;q23) is one of the most common genetic aberrations and results in the formation of the MLL-AF9 fusion gene that functions as a facilitator of cell growth directly. In order to study this type of AML, the cell lines with cytogenetically diagnosed t(9;11)(p22;q23), such as Mono Mac 6 (MM6), have been widely used. To examine whether there is any difference in gene expression between the primary human t(9;11) AML cells and MM6 cell line, genome-wide transcriptome analysis was performed on MM6 cell line using SAGE and the results were compared to the profile of primary human t(9;11) AML cells. 884 transcripts which were alternatively expressed between MM6 cells and primary human t(9;11) cells were identified through statistical analysis (P < 0.05) and 4-fold expression change. Of these transcripts, 830 (94%) matched to known genes or EST were classified by functional categories (http://david.abcc.ncifcrf.gov/). The majority of alternatively expressed genes in MM6 were involved in biosynthetic and metabolic processes, but HRAS, a protein that is known to be associated with leukemogenesis, was expressed only in MM6 cells and several other genes involved in Erk1/Erk2 MAPK pathway were also over-expressed in MM6. Therefore, since MM6 cell line has a similar expression profile to primary human t(9;11) AML in general and expresses uniquely a strong Erk1/Erk2 MAPK pathway including HRAS, it can be used as a model for HRAS-positive t(9;11) AML. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | GENETICS SOC JAPAN | - |
dc.subject | ACUTE MYELOID-LEUKEMIA | - |
dc.subject | RECEPTOR | - |
dc.subject | TRANSLOCATIONS | - |
dc.subject | CYCLOPHILIN | - |
dc.subject | MONO-MAC-6 | - |
dc.subject | GROWTH | - |
dc.subject | SAGE | - |
dc.subject | MLL | - |
dc.title | A comparison of gene expression profiles between primary human AML cells and AML cell line | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, So-Young | - |
dc.identifier.doi | 10.1266/ggs.83.339 | - |
dc.identifier.scopusid | 2-s2.0-54249148932 | - |
dc.identifier.wosid | 000261872300006 | - |
dc.identifier.bibliographicCitation | GENES & GENETIC SYSTEMS, v.83, no.4, pp.339 - 345 | - |
dc.relation.isPartOf | GENES & GENETIC SYSTEMS | - |
dc.citation.title | GENES & GENETIC SYSTEMS | - |
dc.citation.volume | 83 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 339 | - |
dc.citation.endPage | 345 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | ACUTE MYELOID-LEUKEMIA | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | TRANSLOCATIONS | - |
dc.subject.keywordPlus | CYCLOPHILIN | - |
dc.subject.keywordPlus | MONO-MAC-6 | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | SAGE | - |
dc.subject.keywordPlus | MLL | - |
dc.subject.keywordAuthor | AML | - |
dc.subject.keywordAuthor | gene expression | - |
dc.subject.keywordAuthor | SAGE | - |
dc.subject.keywordAuthor | translocation | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.