A comparison of gene expression profiles between primary human AML cells and AML cell line
- Authors
- Lee, Jinseok; Hwang, Junmo; Kim, Hyung-Soo; Kim, Seonggon; Kim, Young Hun; Park, So-Young; Kim, Kil Soo; Ryoo, Zae Young; Chang, Kyu-Tae; Lee, Sanggyu
- Issue Date
- 8월-2008
- Publisher
- GENETICS SOC JAPAN
- Keywords
- AML; gene expression; SAGE; translocation
- Citation
- GENES & GENETIC SYSTEMS, v.83, no.4, pp.339 - 345
- Indexed
- SCIE
SCOPUS
- Journal Title
- GENES & GENETIC SYSTEMS
- Volume
- 83
- Number
- 4
- Start Page
- 339
- End Page
- 345
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122938
- DOI
- 10.1266/ggs.83.339
- ISSN
- 1341-7568
- Abstract
- In acute myeloid leukemia (AML), hematologic malignancies are characterized by recurring chromosomal abnormalities. Chromosome translocation t(9;11)(p22;q23) is one of the most common genetic aberrations and results in the formation of the MLL-AF9 fusion gene that functions as a facilitator of cell growth directly. In order to study this type of AML, the cell lines with cytogenetically diagnosed t(9;11)(p22;q23), such as Mono Mac 6 (MM6), have been widely used. To examine whether there is any difference in gene expression between the primary human t(9;11) AML cells and MM6 cell line, genome-wide transcriptome analysis was performed on MM6 cell line using SAGE and the results were compared to the profile of primary human t(9;11) AML cells. 884 transcripts which were alternatively expressed between MM6 cells and primary human t(9;11) cells were identified through statistical analysis (P < 0.05) and 4-fold expression change. Of these transcripts, 830 (94%) matched to known genes or EST were classified by functional categories (http://david.abcc.ncifcrf.gov/). The majority of alternatively expressed genes in MM6 were involved in biosynthetic and metabolic processes, but HRAS, a protein that is known to be associated with leukemogenesis, was expressed only in MM6 cells and several other genes involved in Erk1/Erk2 MAPK pathway were also over-expressed in MM6. Therefore, since MM6 cell line has a similar expression profile to primary human t(9;11) AML in general and expresses uniquely a strong Erk1/Erk2 MAPK pathway including HRAS, it can be used as a model for HRAS-positive t(9;11) AML.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.