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Selective enrichment of cysteine-containing peptides using SPDP-Functionalized superparamagnetic Fe3O4@SiO2 nanoparticles: Application to comprehensive proteomic profiling

Authors
Palani, ArudraLee, Je-SunHuh, JiyoungKim, MinsikLee, Yeon-JiChang, Jeong HoLee, KwangyeolLee, Sang-Won
Issue Date
8월-2008
Publisher
AMER CHEMICAL SOC
Keywords
superparamagnetic nanoparticle; surface modification; cysteine containing peptide enrichment; tandem mass spectrometry; proteomics
Citation
JOURNAL OF PROTEOME RESEARCH, v.7, no.8, pp.3591 - 3596
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF PROTEOME RESEARCH
Volume
7
Number
8
Start Page
3591
End Page
3596
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/122951
DOI
10.1021/pr800067x
ISSN
1535-3893
Abstract
Superparamagnetic Fe3O4@SiO2 core-shell nanoparticles (ca. 30 nm diameter), which are surface-modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.
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