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Identification and characterization of new microRNAs from pig

Authors
Kim, JungCho, Ik SangHong, Jae SangChoi, Young KiKim, HyunggeeLee, Young Sik
Issue Date
Aug-2008
Publisher
SPRINGER
Citation
MAMMALIAN GENOME, v.19, no.7-8, pp.570 - 580
Indexed
SCIE
SCOPUS
Journal Title
MAMMALIAN GENOME
Volume
19
Number
7-8
Start Page
570
End Page
580
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/122984
DOI
10.1007/s00335-008-9111-3
ISSN
0938-8990
Abstract
MicroRNAs (miRNAs) are small regulatory RNAs that direct the posttranscriptional repression of cognate messenger RNAs. Despite increasing evidence for diverse roles of miRNAs in biological processes, little is known about miRNAs in pig. We describe the first experimental identification of porcine miRNAs by sequence analysis of a cDNA library of small RNAs from porcine fibroblast cells. We identified 25 distinct porcine miRNAs, of which 19 are previously unreported, and define 14 new miRNA families in pig. Most of the cloned miRNAs are expressed ubiquitously in all porcine tissues examined, whereas some miRNAs are expressed preferentially in specific tissues. Our results enrich the porcine miRNA database and provide useful information for investigating biological functions of miRNAs in pig.
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