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High cell density fermentation of Saccharomyces cerevisiae JUL3 in fed-batch culture for the production of beta-glucan

Authors
Kim, Young-HwanKang, Seong WooLee, Jong HoChang, Hyo-IhlYun, Cheol-WonPaik, Hyun-DongKang, Chang-WonKim, Seung Wook
Issue Date
Jan-2007
Publisher
ELSEVIER SCIENCE INC
Keywords
high-cell-density fermentation; Saccharomyces cerevisiae JUL3; molasses; response surface methodology; fed-batch culture
Citation
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.13, no.1, pp.153 - 158
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
Volume
13
Number
1
Start Page
153
End Page
158
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123086
ISSN
1226-086X
Abstract
beta-Glucan is a cell wall component that is one of the most plentiful cell polysaccharides. Moreover, it has been found to have several beneficial effects on the immune system. In yeast, beta-glucan is mainly contained in the yeast cell wall, and thus it is important to produce high levels of cell mass for the mass production of yeast beta-glucan. Response surface methodology (RSM) offers a potential means of optimizing process factors and medium components; it has been used to estimate the effects of medium components on cell mass production. In the present study, the optimal concentrations of molasses and corn steep liquor (CSL) in the medium were determined to be 6.4 % (v/v) and 17 % (v/v). The cell mass predicted by statistical analysis was 9.76 g/L after 20 h of cultivation. In a 2.5-L stirred tank reactor (STR), the cell mass produced in a batch culture was 36.5 similar to 39.3 g/L. The maximum cell mass in the fed-batch cultures of Saccharomyces cerevisiae JUL3 was 95.7 g/L using 50 % molasses solution and a feed rate of 10 mL/h. The cell mass obtained in the fed-batch culture was 2.4-fold higher than that obtained in the batch culture.
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