Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Prostaglandin E-2 augments IL-10 signaling and function

Authors
Cheon, HyeonJooRho, Young HeeChoi, Seong JaeLee, Young HoSong, Gwan GyuSohn, JeongwonWon, Nam HeeJi, Jong Dae
Issue Date
15-Jul-2006
Publisher
AMER ASSOC IMMUNOLOGISTS
Citation
JOURNAL OF IMMUNOLOGY, v.177, no.2, pp.1092 - 1100
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF IMMUNOLOGY
Volume
177
Number
2
Start Page
1092
End Page
1100
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123125
DOI
10.4049/jimmunol.177.2.1092
ISSN
0022-1767
Abstract
In inflamed joints of rheumatoid arthritis, PGE(2) is highly expressed, and IL-10 and IL-6 are also abundant. PGE(2) is a well-known activator of the cAMP signaling pathway, and there is functional cross-talk between cAMP signaling and the Jak-STAT signaling pathway. In this study, we evaluated the modulating effect of PGE(2) on STAT signaling and its biological function induced by IL-10 and IL-6, and elucidated its mechanism in THP-1 cells. STAT phosphorylation was determined by Western blot, and gene expression was analyzed using real-time PCR. Pretreatment with PGE(2) significantly augmented IL-10-induced STAT3 and STAT1. phosphorylation, as well as suppressors of cytokine signaling 3 (SOCS3) and IL-IR antagonist gene expression. In contrast, PGE(2) suppressed IL-6-induced phosphorylation of STAT3 and STAT1. These PGE(2)-induced modulating effects were largely reversed by actinomycin D. Pretreatment with dibutyryl cAMP augmented IL-10-induced, but did not change IL-6-induced STAT3 phosphorylation. Misoprostol, an EP2/3/4 agonist, and butaprost, an EP2 agonist, augmented IL-10-induced STAT3 phosphorylation and SOCS3 gene expression, but sulprostone, an EP1/3 agonist, had no effect. H89, a protein kinase A inhibitor, and LY294002, a PI3K inhibitor, diminished PGE(2)-mediated augmentation of IL-10-induced STAT3 phosphorylation. In this study, we found that PGE2 selectively regulates cytokine signaling via increased intracellular cAMP levels and de novo gene expression, and these modulating effects may be mediated through EP2 or EP4 receptors. PGE(2) may modulate immune responses by alteration of cytokine signaling in THP-1 cells.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Medicine > Department of Medical Science > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Ji, Jong Dae photo

Ji, Jong Dae
College of Medicine (Department of Medical Science)
Read more

Altmetrics

Total Views & Downloads

BROWSE