Macrophage-stimulating activity of exo-biopolymer from cultured rice bran with Monascus pilosus
DC Field | Value | Language |
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dc.contributor.author | Yu, KW | - |
dc.contributor.author | Kim, YS | - |
dc.contributor.author | Shin, KS | - |
dc.contributor.author | Kim, JM | - |
dc.contributor.author | Suh, HJ | - |
dc.date.accessioned | 2021-09-09T06:50:41Z | - |
dc.date.available | 2021-09-09T06:50:41Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2005-07 | - |
dc.identifier.issn | 0273-2289 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123230 | - |
dc.description.abstract | To find a new use of rice bran, five fungi were examined for the production of exo-biopolymer with macrophage-stimulating activity from rice bran. Among the exo-biopolymers produced from the cultures, Monascus pilosus had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve the yield of exo-biopolymer with macrophage-stimulating activity, a suitable medium for exo-biopolymer was tested in submerged culture of M. pilosus. The highest amount of exo-biopolymer (13.9 mg/mL) was obtained in a medium containing rice bran as an only carbon source followed by media with additional maltose and sucrose (13.8 and 13.7 mg/mL, respectively). The addition of peptone resulted in the production of high amount of exo-biopolymer (15.1 mg/mL), meanwhile the addition of ammonium chloride resulted in 264.0 mu g/mL of glucosamine content. Among eight different kinds of inorganic salts tested, potassium phosphate (0.1%) was the most effective inorganic salt for the mycelial growth and exo-biopolymer production. Therefore the optimal medium composition was as follows (g/L): 20 g of rice bran, 5 g of peptone, and 1 g of KH2PO4. The optimal culture pH and time for mycelial growth and exo-biopolymer production was pH 5.0 and 25 degrees C, respectively. The maximum exo-biopolymer (20.1 mg/mL) was observed at the fourth day of cultivation. Exo-biopolymer, a crude polysaccharide fraction, mainly contained neutral sugar (81.8%) with considerable amounts of uronic acid (18.2%). Component sugar analysis showed that the active fraction consisted mainly of arabinose, galactose, glucose, which was digested from starch of rice bran during cultivation, and uronic acid (molar ratio; 0.8:1.0:0.7:0.8). | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | HUMANA PRESS INC | - |
dc.subject | SUBMERGED CULTURE | - |
dc.subject | POLYSACCHARIDES | - |
dc.subject | OPTIMIZATION | - |
dc.subject | PIGMENTS | - |
dc.subject | ACIDS | - |
dc.title | Macrophage-stimulating activity of exo-biopolymer from cultured rice bran with Monascus pilosus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Suh, HJ | - |
dc.identifier.doi | 10.1007/s12010-005-0004-6 | - |
dc.identifier.scopusid | 2-s2.0-22344441301 | - |
dc.identifier.wosid | 000230687500004 | - |
dc.identifier.bibliographicCitation | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.126, no.1, pp.35 - 48 | - |
dc.relation.isPartOf | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.title | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | - |
dc.citation.volume | 126 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 35 | - |
dc.citation.endPage | 48 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | SUBMERGED CULTURE | - |
dc.subject.keywordPlus | POLYSACCHARIDES | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | PIGMENTS | - |
dc.subject.keywordPlus | ACIDS | - |
dc.subject.keywordAuthor | macrophage-stimulating activity | - |
dc.subject.keywordAuthor | Monascus pilosus | - |
dc.subject.keywordAuthor | rice bran | - |
dc.subject.keywordAuthor | submerged culture | - |
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