DNA damage in T and B lymphocytes, bone marrow, spleens, and livers of rats exposed to benzene
- Authors
- Lee, E; Im, H; Oh, E; Jung, WW; Kang, HS; Sul, D
- Issue Date
- 6월-2005
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- DNA damage; benzene
- Citation
- INHALATION TOXICOLOGY, v.17, no.7-8, pp.401 - 408
- Indexed
- SCIE
SCOPUS
- Journal Title
- INHALATION TOXICOLOGY
- Volume
- 17
- Number
- 7-8
- Start Page
- 401
- End Page
- 408
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/123235
- DOI
- 10.1080/08958370590929529
- ISSN
- 0895-8378
- Abstract
- Single-cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the T and B lymphocytes, spleens, bone marrow, and livers of rats exposed to benzene at a concentration of 100, 200, or 400 ppm for 2 or 4 wk. The level of t,t-muconic acid (t,t-MA), which is a urinary benzene metabolite, was determined. In the control rats, mean Olive tail moments in the T and B lymphocytes were 1.507 +/- 0.398 and 1.579 +/- 0.206, respectively. DNA damage in the T and B lymphocytes exposed to 400 ppm benzene for 4 wk caused those rats to exhibit the highest Olive tail moments, with their values measured as 4.351 +/- 0.510 and 3.140 +/- 0.631, respectively. Also, the t,t-MA levels increased directly with increasing benzene exposure time and dose during the 4 wk. After 4 wk, the levels of t,t-MA in urine from rats exposed to 100, 200, and 400 ppm were 19.30 +/- 5.62, 30.36 +/- 4.46, and 46.93 +/- 9.10 mg/g creatinine. In conclusion, the present study demonstrates that benzene exposure results in significant DNA damage in the T and B lymphocytes, bone marrow, spleens, and livers of rats. DNA damage in the blood cells and organs was also discovered to vary directly with benzene exposure, in both a dose-dependent and time-dependent manner. In addition, a similar trend regarding DNA damage was found in the blood cells and organs, and evidenced a good association with the level of t,t-MA in the urine.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.