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Proteomic analysis of human proximal tubular cells exposed to high glucose concentrations

Authors
So, Eun-JeongKim, Hyun-JungKim, Chan-Wha
Issue Date
Jul-2008
Publisher
WILEY-V C H VERLAG GMBH
Keywords
2-DE; diabetic nephropathy; HK-2 cells
Citation
PROTEOMICS CLINICAL APPLICATIONS, v.2, no.7-8, pp.1118 - 1126
Indexed
SCIE
SCOPUS
Journal Title
PROTEOMICS CLINICAL APPLICATIONS
Volume
2
Number
7-8
Start Page
1118
End Page
1126
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123332
DOI
10.1002/prca.200780151
ISSN
1862-8346
Abstract
Hyperglycemia is a major key factor in the pathogenesis of microvascular complications of diabetes, including diabetic nephropathy (DN). Most studies to date have focused on the glomerular abnormalities found in DN. However, nephromegaly in the early stages of diabetes and the correlation of tubulointerstitial pathology rather than glomerular pathology with declining renal function in DN suggests the involvement of the tubulointerstitium. The etiology of the tubulointerstitial pathology in DN, however, is not fully understood. in this study, to understand the DN pathways, we constructed an initial 2-DE reference map for primitively cultured human proximal tubule (HK-2) cell in the presence of 5 mM and 25 mM glucose, which correspond to blood glucose concentrations during the normal and hyperglycemia conditions, respectively. Differentially expressed HK-2 cell cellular proteins at the high glucose concentration were identified via ESI-Q-TOF MS/MS and confirmed by Western blotting; enolase 1 (up-regulated) and lactate dehydrogenase (down-regulated). The regulation of these proteins will help in understanding DN mechanism through the glycolysis metabolic pathways in high glucose stimulated HK-2 cells.
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