Identification of LDL-receptor transcription stimulating peptides from soybean hydrolysate in human hepatocytes
DC Field | Value | Language |
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dc.contributor.author | Cho, Seong-Jun | - |
dc.contributor.author | Juillerat, Marcel A. | - |
dc.contributor.author | Lee, Cherl-Ho | - |
dc.date.accessioned | 2021-09-09T07:21:49Z | - |
dc.date.available | 2021-09-09T07:21:49Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-06-25 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123360 | - |
dc.description.abstract | Soybean protein and its hydrolysate have been reported to have cholesterol-lowering property, but the responsible components are still largely unknown. In previous study, we found that soybean protein hydrolysate (SPH) prepared with the protease from Bacillus amyloliquefaciens FSE-68, strongly stimulates transcription of low density lipoprotein receptor (LDL-R). To identify LDL-R transcription stimulating peptides in human hepatocytes, the SPH was fractionated with gel permeation chromatograpy and the active fraction was further separated by using reverse-phase chromatography. Several peptides in the most active fraction were identified by LC/MS and MS/MS analysis. LDL-R transcription stimulating peptides were synthesized on the basis of identified sequences, and their effect on LDL-R transcription was tested in vitro. Among the synthesized peptides, Phe-Val-Val-Asn-Ala-Thr-Ser-Asn (FVVNATSN) showed the strongest activity, and LDL-R transcription of hepatic cells was increased to 248.8% (compared to 100% of untreated control) by FVVNATSN at a concentration of 100 mu M. This study provides direct evidence that peptides derived from soybean protein can influence LDL-R transcription in hepatocytes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | CORONARY-HEART-DISEASE | - |
dc.subject | CHOLESTEROL HOMEOSTASIS | - |
dc.subject | CARDIOVASCULAR-DISEASE | - |
dc.subject | MYOCARDIAL-INFARCTION | - |
dc.subject | SERUM-CHOLESTEROL | - |
dc.subject | 7S GLOBULIN | - |
dc.subject | CELLS | - |
dc.subject | DENSITY | - |
dc.subject | RISK | - |
dc.subject | DEGRADATION | - |
dc.title | Identification of LDL-receptor transcription stimulating peptides from soybean hydrolysate in human hepatocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Cherl-Ho | - |
dc.identifier.doi | 10.1021/jf800676a | - |
dc.identifier.scopusid | 2-s2.0-47349094769 | - |
dc.identifier.wosid | 000256893200014 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.56, no.12, pp.4372 - 4376 | - |
dc.relation.isPartOf | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 56 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 4372 | - |
dc.citation.endPage | 4376 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | CORONARY-HEART-DISEASE | - |
dc.subject.keywordPlus | CHOLESTEROL HOMEOSTASIS | - |
dc.subject.keywordPlus | CARDIOVASCULAR-DISEASE | - |
dc.subject.keywordPlus | MYOCARDIAL-INFARCTION | - |
dc.subject.keywordPlus | SERUM-CHOLESTEROL | - |
dc.subject.keywordPlus | 7S GLOBULIN | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | DENSITY | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordAuthor | soybean | - |
dc.subject.keywordAuthor | bloactive peptide | - |
dc.subject.keywordAuthor | cholesterol | - |
dc.subject.keywordAuthor | LDL-receptor | - |
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