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Expressions of Caspase-14 in Human Middle Ear Cholesteatoma

Authors
Jung, Myung-HoLee, Jang HyeogCho, Jae-GuJung, Hak HyunHwang, Soon-JaeChae, Sung-Won
Issue Date
Jun-2008
Publisher
LIPPINCOTT WILLIAMS & WILKINS
Keywords
Cholesteatoma; caspase-14
Citation
LARYNGOSCOPE, v.118, no.6, pp.1047 - 1050
Indexed
SCIE
SCOPUS
Journal Title
LARYNGOSCOPE
Volume
118
Number
6
Start Page
1047
End Page
1050
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123440
DOI
10.1097/MLG.0b013e3181671b4d
ISSN
0023-852X
Abstract
Objectives: Cholesteatoma is characterized by an excessive proliferation and differentiation of keratinocytes with a progressive accumulation of keratin debris. Caspase-14 is a novel regulator of keratinocyte terminal differentiation. The purpose of this study was to investigate the expression patterns and localizations of caspase-14 in cholesteatoma and in normal external auditory canal (EAC) epithelium. Methods: The expression levels of caspase-14 mRNA were evaluated through real-time polymerase chain reaction (PCR) and Western blotting. Cholesteatoma and normal EAC epithelium were immunostained with a monoclonal antibody to caspase-14. The localizations of immunoreactivity to the caspase-14 antibody were compared between cholesteatoma and normal EAC epithelium through inummohistochemical staining. Results: As shown by real-time reverse-transcription PCR, the expression level of caspase-14 mRNA in cholesteatoma epithelium was significantly higher than in normal EAC epithelium. Caspase-14 protein was detected in both normal EAC and cholesteatoma, but its expression was shown to be greater in cholesteatoma on Western blot analysis. As shown with immunohistochemical staining, caspase-14 protein was primarily expressed in the granular layer and the upper parts of the spinous layer in cholesteatoma epithelium and in the superficial layer of normal EAC epithelium. The expression of caspase-14 was more intense in cholesteatoma tissues than in normal EAC epithelium. Conclusion: The increased level of caspase-14 expression in cholesteatoma tissues may play a role in terminal differentiation of epithelium and accumulation of keratin debris from external matrix.
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