The neuronal differentiation potential of Ldb1-null mutant embryonic stem cells is dependent on extrinsic influences
- Authors
- Hwang, Minyoung; Gorivodsky, Marat; Kim, Minjung; Westphal, Heiner; Geum, Dongho
- Issue Date
- 6월-2008
- Publisher
- WILEY
- Keywords
- Ldb1; embryonic stem cells; neuronal differentiation; embryoid body; five-stage method; adherent monolayer culture method
- Citation
- STEM CELLS, v.26, no.6, pp.1490 - 1495
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELLS
- Volume
- 26
- Number
- 6
- Start Page
- 1490
- End Page
- 1495
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/123475
- DOI
- 10.1634/stemcells.2007-1099
- ISSN
- 1066-5099
- Abstract
- LIM-domain binding protein 1 (Ldb1) is a multiadaptor protein that mediates the action of transcription factors, including LIM-homeodomain proteins. To elucidate the functional role of Ldb1 in the neuronal differentiation of embryonic stem (ES) cells, we have generated Ldb1-null mutant (Ldb1(-/-)) ES cells and examined neuronal differentiation potentials in vitro using two different neuronal differentiation protocols. When subjected to a five-stage protocol that recapitulates in vivo conditions of neuronal differentiation, wild-type ES cells differentiated into a wide spectrum of neuronal cell types. However, Ldb1(-/-) ES cells did not differentiate into neuronal cells; instead, they differentiated into sarcomeric alpha-actinin-positive muscle cells. In contrast, when an adherent monolayer culture procedure (which is based on the default mechanism of neural induction and eliminates environmental influences) was applied, both wild-type and Ldb1(-/-) ES cells differentiated into MAP2-positive mature neurons. Comparison of the results obtained when two different neuronal differentiation protocols were used suggests that Ldb1(-/-) ES cells have an innate potential to differentiate into neuronal cells, but this potential can be inhibited by environmental influences.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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