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Bone marrow-derived side population cells are capable of functional cardiomyogenic differentiation

Authors
Yoon, JihyunChoi, Seung-CheolPark, Chi-YeonChoi, Ji-HyunKim, Yang-InShim, Wan-JooLim, Do-Sun
Issue Date
30-Apr-2008
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Keywords
bone marrow; cardiomyogenic differentiation; side population cells
Citation
MOLECULES AND CELLS, v.25, no.2, pp.216 - 223
Indexed
SCIE
SCOPUS
KCI
Journal Title
MOLECULES AND CELLS
Volume
25
Number
2
Start Page
216
End Page
223
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123712
ISSN
1016-8478
Abstract
It has been reported that bone marrow (BM)-side population (SP) cells, with hematopoietic stem cell activity, can transdifferentiate into cardiomyocytes and contribute to myocardial repair. However, this has been questioned by recent studies showing that hematopoietic stem cells (HSCs) adopt a hematopoietic cell lineage in the ischemic myocardium. The present study was designed to investigate whether BM-SP cells can in fact transdifferentiate into functional cardiomyocytes. Phenotypically, BM-SP cells were 19.59% +/- 9.00 CD14(+), 8.22% +/- 2.72 CD34(+), 92.93% +/- 2.68 CD44(+), 91.86% +/- 4.07 CD45(+), 28.48% +/- 2.24 c-kit(+), 71.09% +/- 3.67 Sca-1(+). Expression of endothelial cell markers (CD31, Flk-1, Tie-2 and VEGF-A) was higher in BM-SP cells than whole BM cells. After five days of co-culture with neonatal cardiomyocytes, 7.2% +/- 1.2 of the BM-SP cells expressed sarcomeric alpha-actinin as measured by flow cytometry. Moreover, BM-SP cells co-cultured on neonatal cardiomyocytes fixed to inhibit cell fusion also expressed sarcomeric alpha-actinin. The co-cultured BM-SP cells showed neonatal cardiomyocyte-like action potentials of relatively long duration and shallow resting membrane potential. They generated calcium transients with amplitude and similar to those of neonatal cardiomyocytes. results show that BM-SP cells are capable of cardiomyogenic differentiation when co-cultured with neonatal cardiomyocytes.
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