ssDNA aptamer-based surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes
DC Field | Value | Language |
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dc.contributor.author | Lee, Su Jin | - |
dc.contributor.author | Youn, Byung-Soo | - |
dc.contributor.author | Park, Ji Woo | - |
dc.contributor.author | Niazi, Javed H. | - |
dc.contributor.author | Kim, Yeon Seok | - |
dc.contributor.author | Gu, Man Bock | - |
dc.date.accessioned | 2021-09-09T09:15:01Z | - |
dc.date.available | 2021-09-09T09:15:01Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-04-15 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/123727 | - |
dc.description.abstract | Retinol binding protein 4 (RBP4) is a useful biomarker in the diagnosis of type 2 diabetes since its level in the serum is higher in insulin-resistant states. Accurate measurement of the serum RBP4 levels is hampered by conventional immunologic methods, such as enzyme-linked immunosorbent assay (ELISA). In this study, therefore, we have developed an aptamer-based surface plasmon resonance (SPR) biosensor that can be used to sense for RBP4 in serum samples. A single-stranded DNA (ssDNA) aptamer that showed high affinity (K-d = 0.2 +/- 0.03 mu M) and specificity to RBP4 was selected. This RBP4-specific aptamer was immobilized on a gold chip and used in a label-free RBP4 detection using SPR. Analysis of RBP4 in artificial serum using SPR was compared with ELISA and Western blot analysis. Our results indicated that the RBP4-specific aptamer-based SPR biosensor gave better dose-dependent responses and was more sensitive than ELISA assays. As such, this RBP4 aptamer-based SPR biosensor can be potentially used to monitor the RBP4 levels within the serum as an indicator of type 2 diabetes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | INSULIN-RESISTANCE | - |
dc.subject | BINDING | - |
dc.subject | OBESITY | - |
dc.subject | MOLECULES | - |
dc.subject | SELECTION | - |
dc.subject | PROTEIN | - |
dc.subject | SERUM | - |
dc.title | ssDNA aptamer-based surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gu, Man Bock | - |
dc.identifier.doi | 10.1021/ac800050a | - |
dc.identifier.scopusid | 2-s2.0-42349091244 | - |
dc.identifier.wosid | 000254969100029 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.80, no.8, pp.2867 - 2873 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 80 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2867 | - |
dc.citation.endPage | 2873 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | SERUM | - |
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