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ssDNA aptamer-based surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes

Authors
Lee, Su JinYoun, Byung-SooPark, Ji WooNiazi, Javed H.Kim, Yeon SeokGu, Man Bock
Issue Date
15-4월-2008
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.80, no.8, pp.2867 - 2873
Indexed
SCIE
SCOPUS
Journal Title
ANALYTICAL CHEMISTRY
Volume
80
Number
8
Start Page
2867
End Page
2873
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/123727
DOI
10.1021/ac800050a
ISSN
0003-2700
Abstract
Retinol binding protein 4 (RBP4) is a useful biomarker in the diagnosis of type 2 diabetes since its level in the serum is higher in insulin-resistant states. Accurate measurement of the serum RBP4 levels is hampered by conventional immunologic methods, such as enzyme-linked immunosorbent assay (ELISA). In this study, therefore, we have developed an aptamer-based surface plasmon resonance (SPR) biosensor that can be used to sense for RBP4 in serum samples. A single-stranded DNA (ssDNA) aptamer that showed high affinity (K-d = 0.2 +/- 0.03 mu M) and specificity to RBP4 was selected. This RBP4-specific aptamer was immobilized on a gold chip and used in a label-free RBP4 detection using SPR. Analysis of RBP4 in artificial serum using SPR was compared with ELISA and Western blot analysis. Our results indicated that the RBP4-specific aptamer-based SPR biosensor gave better dose-dependent responses and was more sensitive than ELISA assays. As such, this RBP4 aptamer-based SPR biosensor can be potentially used to monitor the RBP4 levels within the serum as an indicator of type 2 diabetes.
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