ssDNA aptamers that selectively bind oxytetracycline
DC Field | Value | Language |
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dc.contributor.author | Niazi, Javed H. | - |
dc.contributor.author | Lee, Su Jin | - |
dc.contributor.author | Kim, Yeon Seok | - |
dc.contributor.author | Gu, Man Bock | - |
dc.date.accessioned | 2021-09-09T11:25:38Z | - |
dc.date.available | 2021-09-09T11:25:38Z | - |
dc.date.created | 2021-06-10 | - |
dc.date.issued | 2008-02-01 | - |
dc.identifier.issn | 0968-0896 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/124103 | - |
dc.description.abstract | Single stranded DNA aptamers that bind with high affinity and specificity to the oxytetracycline (OTC) were identified by selection from an oligonucleotide library of 1 015 molecules. The binding affinities of four aptamers were in nanomolar range. The aptamers were highly selective in that, lack of -OH group at 5-position in tetracycline and -H group in place of -OH at 6-position in doxycycline determined the specificity of these aptamers to bind OTC. Three aptamers designated as No. 4, 5, and 20 shared strong affinities with K-d = 9.61, 12.08, and 56.84 nM, respectively, as well as selectivity to bind OTC (72-76%). Aptamer No. 4 had strong affinity among all with high selectivity, whereas No. 2 had relatively weak affinity (K-d = 121.1 nM) and moderate selectivity (52%). Our results indicated that the aptamers No. 4, 5, and 20 with variable 40-base oligonucleotides can be good candidates for selectively binding to OTC with high molecular discrimination over its analogs such as tetracycline and doxycycline. (c) 200 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | ELECTROCHEMICAL DETECTION | - |
dc.subject | LIQUID-CHROMATOGRAPHY | - |
dc.subject | TETRACYCLINE | - |
dc.subject | RNA | - |
dc.subject | ANTIBIOTICS | - |
dc.title | ssDNA aptamers that selectively bind oxytetracycline | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gu, Man Bock | - |
dc.identifier.doi | 10.1016/j.bmc.2007.10.073 | - |
dc.identifier.scopusid | 2-s2.0-38849186073 | - |
dc.identifier.wosid | 000253720100019 | - |
dc.identifier.bibliographicCitation | BIOORGANIC & MEDICINAL CHEMISTRY, v.16, no.3, pp.1254 - 1261 | - |
dc.relation.isPartOf | BIOORGANIC & MEDICINAL CHEMISTRY | - |
dc.citation.title | BIOORGANIC & MEDICINAL CHEMISTRY | - |
dc.citation.volume | 16 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1254 | - |
dc.citation.endPage | 1261 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.subject.keywordPlus | ELECTROCHEMICAL DETECTION | - |
dc.subject.keywordPlus | LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | TETRACYCLINE | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | ANTIBIOTICS | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Oxytetracycline | - |
dc.subject.keywordAuthor | SELEX | - |
dc.subject.keywordAuthor | ssDNA | - |
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