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ssDNA aptamers that selectively bind oxytetracycline

Authors
Niazi, Javed H.Lee, Su JinKim, Yeon SeokGu, Man Bock
Issue Date
1-2월-2008
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
Aptamer; Oxytetracycline; SELEX; ssDNA
Citation
BIOORGANIC & MEDICINAL CHEMISTRY, v.16, no.3, pp.1254 - 1261
Indexed
SCIE
SCOPUS
Journal Title
BIOORGANIC & MEDICINAL CHEMISTRY
Volume
16
Number
3
Start Page
1254
End Page
1261
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/124103
DOI
10.1016/j.bmc.2007.10.073
ISSN
0968-0896
Abstract
Single stranded DNA aptamers that bind with high affinity and specificity to the oxytetracycline (OTC) were identified by selection from an oligonucleotide library of 1 015 molecules. The binding affinities of four aptamers were in nanomolar range. The aptamers were highly selective in that, lack of -OH group at 5-position in tetracycline and -H group in place of -OH at 6-position in doxycycline determined the specificity of these aptamers to bind OTC. Three aptamers designated as No. 4, 5, and 20 shared strong affinities with K-d = 9.61, 12.08, and 56.84 nM, respectively, as well as selectivity to bind OTC (72-76%). Aptamer No. 4 had strong affinity among all with high selectivity, whereas No. 2 had relatively weak affinity (K-d = 121.1 nM) and moderate selectivity (52%). Our results indicated that the aptamers No. 4, 5, and 20 with variable 40-base oligonucleotides can be good candidates for selectively binding to OTC with high molecular discrimination over its analogs such as tetracycline and doxycycline. (c) 200 Elsevier Ltd. All rights reserved.
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