DNA damage in T- and B-lymphocytes and granulocytes in emission inspection and incineration workers exposed to polycyclic aromatic hydrocarbons
- Authors
- Sul, D; Oh, E; Im, H; Yang, M; Kim, CW; Lee, E
- Issue Date
- 8-7월-2003
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- comet assay; dioxin; creatinine; lymphocytes; 1-hydroxypyrene; 2-naphthol
- Citation
- MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v.538, no.1-2, pp.109 - 119
- Indexed
- SCIE
SCOPUS
- Journal Title
- MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
- Volume
- 538
- Number
- 1-2
- Start Page
- 109
- End Page
- 119
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/124354
- DOI
- 10.1016/S1383-5718(03)00095-0
- ISSN
- 1383-5718
- Abstract
- In this study, we investigated by using comet assay the effects of polycyclic aromatic hydrocarbon (PAH) as a major factor on DNA damage of workers exposed to exhaust fumes. Twenty-four workers from three automobile emission inspection companies, 28 workers from a waste incinerating company, and 43 matched, unexposed healthy subjects were enrolled in the study. The mean values of 1-hydroxypyrene (1-OHP) in automobile emission inspection and waste incineration workers were 0.27 +/- 0.19 and 0.57 +/- 0.46 mumol/mol creatinine, respectively, and the mean values of 2-naphthol in automobile emission inspectors and waste incineration workers were 4.80 +/- 4.01 and 8.30 +/- 4.79 mol/mol creatinine, respectively. Significant difference in urinary metabolites, I-hydroxypyrene and 2-naphthol was found between smokers and non-smokers in exposed groups and it may be due to the amounts of smoking cigarettes. In T-lymphocytes, DNA damage in control subjects, emission inspection workers and incineration workers were 1.42 +/- 0.22, 1.41 +/- 0.22 and 1.76 +/- 0.27, respectively. DNA damage of B-lymphocytes in the three groups showed the most significant differences of three cell types. The tail moments of the B-lymphocytes of control subjects, emission inspection and incineration workers were 1.40 +/- 0.27,2.44 +/- 0.32 and 2.36 +/- 0.37, respectively. In granulocytes, DNA damage was also different, the tail moments being 2.72 +/- 0.59,3.32 +/- 0.38 and 2.85 +/- 0.49, respectively. Although 1-OHP and 2-naphthol levels were statistically increased in smokers in workers exposed to PAHs, exposed smoking and non-smoking workers did not show any significantly difference in terms of Olive tail moments. Our results suggest that PAH causes single strand DNA breakage in human T- and B-lymphocytes, and granulocytes. A comparison of DNA damage in three groups showed that B-lymphocytes are useful target in the biomonitoring of human exposure. (C) 2003 Elsevier Science B.V. All rights reserved.
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