Simultaneous quantitation of five flavonoid glycosides in Herba Epimedii by high-performance liquid chromatography-tandem mass spectrometry
- Authors
- Islam, Nurul M.; Yoo, Hye Hyun; Lee, Min Woo; Dong, Mi-Sook; Park, Young In; Jeong, Ha Sook; Kim, Dong-Hyun
- Issue Date
- 1월-2008
- Publisher
- WILEY
- Keywords
- Herba Epimedii; HPLC-MS/MS; quantitative analysis; flavonoid glycosides
- Citation
- PHYTOCHEMICAL ANALYSIS, v.19, no.1, pp.71 - 77
- Indexed
- SCIE
SCOPUS
- Journal Title
- PHYTOCHEMICAL ANALYSIS
- Volume
- 19
- Number
- 1
- Start Page
- 71
- End Page
- 77
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/124547
- DOI
- 10.1002/pca.1018
- ISSN
- 0958-0344
- Abstract
- A rapid and accurate reversed-phase liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for the quantitative determination of five flavonoid glycosides, icariin, epimedin A, epimedin B, epimedin C and hyperin in Herba Epimedii. Chromatographic separations were performed using a C-18 narrow-bore HPLC column; a mixture of an aqueous solution of ammonium formate (pH 4.0) and acetonitrile was used as the mobile phase, with compounds detected in the positive ion mode with multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with an electrospray ionisation interface. This method for the determination of the reported flavonoid glycosides was accurate and reproducible, with a lower limit of quantification of 0.5 mu g/mL. The standard calibration curves for the above-mentioned compounds were linear (r(2) > 0.998) over the concentration range 0.5-10.0 mu g/mL. The relative standard deviations for intra- and inter-day precision over the concentration range for the flavonoid glycosides were lower than 7.8% with accuracy between 90.1 and 111.0%. The established method was successfully applied to the quality assessment of samples of Herba Epimedii collected from Korea and China. Copyright (C) 2007 John Wiley & Sons, Ltd.
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